rebeccmeister: (Default)
When I left Arizona in 2018, I carried along with me a small batch of Palo Brea seeds thinking and hoping that I'd eventually be able to grow some small plants here. (I should note that Palo Brea are fast-growing TREES, so I'd have to cull them after a point or turn them into bonsai). The seeds are still sitting in a small Mason jar here on my desk, at work. I'm not entirely certain of their germination requirements; I believe that, in the wild, they typically germinate during the hot summer monsoon months.

The trouble is, I have run out of dried Palo Brea leaves to feed the two leafcutter colonies here. So, what to do?

Some of the projects from Scientific Writing this past fall gave me an idea. This species seems to enjoy harvesting leaves from leguminous plants. So, maybe I should try growing a leguminous plant or two, and feed the shoots to the ants.

I used my lab's fancy drill press to drill more holes in the bottom of more yogurt tubs, and took advantage of my lab's nice, deep windowsill, plus a set of my favorite green seed trays that I discovered in the supplies I inherited with my lab space, to begin my ExPEAriment, as I am calling it:

Take that, Winter!
ExPEAriment with Acromyrmex versicolor

Initial popularity test:
ExPEAriment with Acromyrmex versicolor

Not the greatest macro photo setup, but hopefully if you squint you can see that the ants are, indeed, adding green leaf chunks to the fungus garden:

ExPEAriment with Acromyrmex versicolor

A couple hours later, and the evidence is clear: they like it!

ExPEAriment with Acromyrmex versicolor

The pea plants continue to grow:
ExPEAriment with Acromyrmex versicolor

So I gave them a bunch more today.

A bonANTza, as my PhD advisor would say!

ExPEAriment with Acromyrmex versicolor

ExPEAriment with Acromyrmex versicolor

ExPEAriment with Acromyrmex versicolor

They like to get internal storage depots going sometimes before adding stuff into the fungus garden:

ExPEAriment with Acromyrmex versicolor

It's fun to watch them at work.

There's actually even more backstory to this. Way back during my PhD I tried to grow and feed wheatgrass to some leafcutter ant colonies, based on the suggestion of one of my PhD advisors who primarily works with grasshoppers. But I could never seem to get the logistics right for the wheatgrass. Instead of a lush carpet of wheatgrass I only ever managed to grow a few spindly blades, and even when I tried giving colonies a handful of wheatgrass hacked from a storebought container, it just didn't seem like I got much plant material. I think that wheatgrass might just be too watery and insubstantial for the ants.

So the pea shoots are looking MUCH more promising, which is even more satisfying in the context of those wheatgrass failures from so long ago.
rebeccmeister: (Acromyrmex)
Monsoon rainfall patterns have been interesting this year. First, there was the rain in June; unusually early for a monsoon rain. We managed to collect 60 leafcutter queens from that rain, from a small swarm: enough to feel like the early morning trip down to Tucson was worthwhile, but not enough for all of the projects we want to do.

The more recent storm system has dropped a whole bunch of rain, but the prevailing winds have caused it to travel and drop that rain basically everywhere else except over our usual main collecting area, on the south side of the Catalina Mountains.

Even so, given all the rain elsewhere, I have been getting concerned that we'd missed out on opportunities to collect leafcutter queens from other places. So that justified a trip down. Looking at my schedule for the next couple of days, I determined that yesterday afternoon was the best time to go. We left at around 4:30, in order to get there while there was still enough daylight left to look around and see what areas might pan out.

Our usual destinations were dry, showing very little signs of activity, so then I suggested we head due east, towards the Seven Falls Trailhead area, where the nearby rain gauge had indicated higher rainfall amounts.

I was able to do some ant-spotting while sitting in the passenger seat as we drove around at dusk with the windows down. When leafcutter queens mate and then find places to dig and start new colonies, they leave distinct craters of soil tailings around their excavation sites, and these are reasonably obvious to someone with an experienced eye. The vegetation and soil near the Seven Falls Trailhead looked like the right kind, and soon enough I also spotted some of the distinctive craters that mature Acromyrmex colonies make. Shortly after that, we found a few patches of ground in a road median that looked promising.

That was enough to justify the entrance price to park at the trailhead, where we continued to walk around through an incredible sunset and the onset of dusk.

From here, I mostly want to note the other awesome wildlife that we saw while queen-hunting, although I'll also note that we managed to come back with 74 queens, which made the whole trip worthwhile. I very randomly caught the first one just after nightfall, in a non-obvious spot, which was an encouraging sign.

First, the tarantula hawks: big ones, at least 2 inches long, actively traveling around and searching at night for tarantulas. They are so beautiful, with their dark blue, shiny bodies, and shiny, red-orange wings. Also slightly terrifying, when you figure that they may fly towards the light from flashlights at night. Then, the tarantulas themselves - we saw at least 3 of them. Tons more mature leafcutter colonies, which will be really useful for some of N's dissertation projects. At least 6 banded geckos. A small Sonoran desert toad, and then a bigger 'un, the size of a softball, that scared me a little with its loud rustling. Lots of kangaroo mice. And then, late into the evening, 4 javelinas trotted close by, following a trail to get from somewhere to somewhere else. My first Arizona javelinas. Also a good number of millipedes, and at least one centipede, and a small snake that tried very hard to be inconspicuous, hiding in its hole.

It was a good collecting trip, and I'm keen to go back and visit that area again sometime. It was beautiful. We got back to Tempe by around 1:00 am, dropped the queens into nests, and so I got to sleep by 2 am.

Drain bed

Jul. 11th, 2018 08:07 pm
rebeccmeister: (Acromyrmex)
"I say, Marge, do you ever get that funny feeling you're being watched?"
Filming ant queen behavior

more photos... )

The Weather

Jul. 9th, 2018 09:13 am
rebeccmeister: (Default)
It's definitely monsoon season now.

Saturday morning, pre-monsoon, [personal profile] scrottie and I got up early and biked over to the Phoenix Farmer's Market. They had their mister system running at full blast, such that it felt like a tropical rainforest. On the return ride, the wind blew hot in our faces. Applying water to the top of my head felt refreshing.

Later in the afternoon, I went out again to run a couple of errands. Before I left, I soaked my linen shirt in water. Putting it on felt like donning a wet bathing suit - clammy, cold. The second I started bicycling, I felt fantastic. That feeling lasted for the entire 5 minutes while the shirt was still wet.

We're now at the point where the "cold" water coming out of the faucet is warm. No need to use hot water while showering.

Last night there were some good showers just north of Tucson, north of the Catalina Mountains, in a part of the state where we were once caught out in a microburst at the very end of a 400k brevet. There are leafcutter ants out there, but I don't know at what sort of density, so we didn't go down.

When the sun came up this morning, the sky was brown-orange. Cars are covered in a fine layer of dust: dust storm. The bike ride in to campus was humid, but at least temperatures were cooler than they have been.

I am hoping I won't have to do too much data collection this week.
rebeccmeister: (Default)
Friday afternoon/evening: Whirlwind grocery store stop after work, made dinner, and fed my sis-in-law and kids. Seitan fajitas! The grown-ups enjoyed them tremendously*. Note to self: get or think of more kid-friendly entertainment stuff for when kids come over to visit. Then, packing for hiking and camping.

Saturday morning: Up early, made coffee and breakfast burritos for myself and brother's family, wedged camping supplies into the car, then wedged myself into the middle seat of their Subaru between two kids in car seats. We drove up towards Oak Creek Canyon, with a quick stop in Sedona for second breakfast*. We reached the parking lot for the West Fork hike at 9:30 am. There were four cars idling in line, and yes, the lot was full. So we backtracked one site to the Bootlegger Picnic Area and splashed in the creek there. Maybe it was for the best, as the small children were only up for about 0.5 miles of hiking anyway.

After lunch, we drove up to the Lava River Cave, which was very popular with the short set AND the adults alike. Primitive camping out in the Coconino National Forest was also a hit. So nice and quiet among the pines. What a relief. C enjoyed getting to watch and listen to some common nighthawks dive-bombing (see/hear an example here), but didn't find any nightjars.

Sunday - Tuesday: Got up, packed up, drove back to Phoenix, said goodbye to my brother and family, ate lunch, jumped in the shower, did some repacking, then loaded gear into another postdoc's car for the 6-hour drive out to Pine Valley, CA. Thankfully her car wasn't as cramped as the Subaru.

The last time I went out to collect harvester ant foundress queens in southern California was over a decade ago, I think in 2007 or 2008. Cibbet Flats was still a nice campground. The big field in Pine Valley was still there. The campground at Lake Henshaw is still sketchy. The pie from Julian is still mighty fine.

The mating flights have definitely shifted earlier over the past decade. The peak used to occur right around the 4th of July; now the flights are mostly done by then. We still managed to come back with 46 queens from the cooperative site and 59 queens from the non-cooperative site. It was a fun group of people to collect and camp with.

The break from typing has been helpful. Back at it soon. Also, it's July already and almost time to start packing things up again. Also time to keep looking for a place to rent.




*Comments about children with extremely picky eating habits deliberately left out.
rebeccmeister: (Default)
Three Fridays ago, [personal profile] scrottie and I did a 45-mile Friday night ride we refer to as "Around the World", which loops north through Scottsdale up to Fountain Hills, then back again. Last weekend, we rode up to Paradise Valley, and on the ride there, had a disagreement about proper cadences and cycling methodology. As a result, this past Friday, I wound up going out to ride the 75-mile "Around the Universe" variant on my own.

This won't matter much to most readers who don't know the area, but the 75-mile version was WAY more pleasant than the 45-mile version. The 45-mile version involves an uphill climb on Shea Blvd, which is a major arterial and lacks bicycling amenities along a key uphill segment. As we were riding along, some dude in a convertible saw fit to try and deliver a lecture on cycling safety to us from the next lane over, which didn't help matters ("Do you have ANY idea of what you're doing?!" Um, yes, dude, yes, we do.). To top that off, the 45-mile version put us back onto Highway 87 at around 10 pm, a time of night when there is still a lot of traffic.

In contrast, the Around the World route avoids Shea by going up and over Dynamite Blvd instead. Sure, it's more climbing, but I just shifted down and worked my way up at a moderate pace. After the descent down Dynamite, the whole section along McDowell Mountain Park was quiet and wonderful, and there were some sections with refreshing pockets of cool air. And by the time I got all the way back down to Highway 87, it was late enough that traffic had really quieted down.

I don't know if I'll wind up building all the way back up to the full Midnight Century or not, because the full thing takes that much more time, late at night, and I'd like to hold on to some semblance of a normal sleep schedule. But the Around the Universe was really nice and it felt good to lay down some miles.

I saw a palo verde beetle on the bike path towards the beginning of the ride. They are so cool.
Palo verde beetle

-

Both ceramics sessions this week were fairly uneventful. Mud therapy.

-

The people in the lab who work with the seed-harvester ants are currently running an "Ant Farm" experiment, which involves putting ants into hundreds of nests like this one and then observing their behavior and social interactions:
Observation farm

The two queens in this photo are excavating: digging up soil, carrying it to the surface in their chin-whisker beards, and depositing it there. Okay, only ~150 nests at the moment. I went in to help for 2 hours on Saturday, but am trying to avoid getting sucked in too much. Not my project.

Afterwords, I ate lunch in the breakroom. I hardly ever eat lunch in there, but the view is phenomenal if you enjoy an industrial aesthetic:

Breakroom view

You can just barely see the tops of the palm trees along Palm Walk. The tallest building in the background is the Life Sciences E wing. The stuff in the foreground is the top of some engineering buildings.

-

I originally thought I'd be spending most of today hanging out with my cousin A, but then she had a change of plans. Unexpected free day got devoted to: baking pumpkin scones, lemon-ricotta muffins, and bread; vacuuming like woah*; a whole series of minor bike tweaks**; and various other odds and ends. Then S and I booked the moving truck for the Great Crazy American Cross-Country Junk-Hauling Expedition. By the dubs - it cost $50 for S to join AAA, and that knocked $500 off the truck rental price (=net $450 savings).



*I don't think I've mentioned too recently that I hate carpeting. I hate carpeting. Also, Arizona is so dusty that I had to empty out the (bagless) vacuum like 12 times while vacuuming this 800-square-foot place. Yes, literally, 12 times.

**Froinlavin got 2 new hot pink bottle cages and is now all matchy-matchy with the black and hot pink aesthetic. The Jolly Roger got a 1.5" rear tire to replace the 1.75" one, a swapped bottle cage to replace one I decided I didn't like (annoying design), some much-needed basket repair work, and new festive basket lights for night riding.

Leaves

Jun. 20th, 2018 06:15 pm
rebeccmeister: (Acromyrmex)
When I arrived at work this morning, the campus landscaping crew was busy pruning the palo brea trees right outside the building.

It took an hour or so before I had that asterisk moment. (kind of like a lightbulb, but not quite.) I can't believe it took that long.

I dragged in two armloads of spiny branches, and spent all my spare moments today stripping off the all the leaves.

Palo brea leaves cause a mild allergic reaction, in addition to the unpleasant finger stabs.

I need to do some leaf nutrient analysis tests, which requires a fairly large/unknown amount of leaf material, which I need to attempt to homogenize/standardize.

So next I need to figure out how I'm going to dry and chop the leaves up. The lab's drying oven failed recently, and I don't know how soon they'll get a replacement.

OH - food dehydrator. Yes, THAT was a lightbulb.

The standard tool for chopping up leaf material is a Wiley cutting mill (do an image search to see). Nobody has one around here. Alternative ideas welcome.

-

Meanwhile, I've been learning about the methods used to quantify leaf fiber content, which involve boiling leaves in various detergents that basically extract out everything and leave the fiber behind. This requires specialized, expensive "Gooch crucibles" for the filtration steps. Science eBay to the rescue.
rebeccmeister: (Acromyrmex)
So I managed to get this first bowl finished during the first ceramics session, as you might recall:

Dragonfly bowl

But I have several more bowls to go. Painting the dragonfly with black iron oxide took a while. Here's how that bowl looked before the glazing and firing:

Dragonfly Bowl 1

At the moment I have a bunch of other projects in the works, too, so I didn't want to spend Saturday's studio time entirely on painting. I do, however, want to continue making these kinds of pieces. So I worked on two more bowls at home on Saturday morning. I'm hesitant to show the outcome of the painting effort for the new bowls before things get fired again, because as you can see from the work above, glaze firing is a dynamic process and things almost never turn out exactly as you expect them to. So we shall see. Then I managed to spend the entire afternoon in the studio making headway on the other projects. A full day of ceramics - not bad. I strategically sat at the wheel that was furthest away from the noisiest classmates, and that made things much more tolerable for the afternoon.

When I first arrived at the studio, another new student had appeared. We took one look at each other and went, "I know you." It was J, someone else who was a regular back when I did ceramics during grad school. It was comforting to see him there. He's kind of funny in that his specialty is cylindrical vases with textural decorations - that's basically the only thing he makes, as far as I can tell. He made two of them during Saturday's class. How many vases does a person need? He says he gives away pretty much all of them. His approach reflects the personal tactile comfort/engagement aspect of ceramics. Clay speaks to different people in different ways.

-

Grad student N and I have been keeping a close eye on the weather over the past week, ever since we'd heard that there would probably be an unusually early monsoon storm coming up. Ant-hunting season has begun. And storm it did, although as with most monsoon rain the amount of rain a given location receives can vary tremendously. We only got a few light sprinkles in Dogtown, but the neighborhoods north of the Phoenix Mountains were more thoroughly drenched. The bulk of the rain fell from Friday night through Saturday morning, which meant that we wouldn't need to go down to Tucson until Sunday morning. This was a small relief, given the ambitious ceramics agenda you can see outlined above.

There was just one slight hitch, in that [personal profile] scrottie and I had made plans to have dinner with friends who live in Paradise Valley on Saturday night. I enjoy bicycling up there because then I get the combination of decent exercise AND quality time with good friends. But all told, it takes about 1.5 hours to ride up there, and another 1.5 hours to ride home. So we didn't get home and into bed until 1:30 am.

I don't think I really slept, because I then had to get up by 3:30 am so N and I could drive down to Tucson in time to catch the leafcutter mating swarms, shortly after sunrise.

We made good time on the drive, arriving by 5:45 am. The total rainfall accumulation in our key collecting area was just borderline for what we think it takes to trigger mating swarms, but if we didn't go down there we would never know whether we'd wind up missing our only opportunity to get queens.

Typically, during a good swarming event, we will drive past multiple mating aggregations on our way out to our main collecting area. Yesterday morning, we saw next to no signs of activity as we drove along. It occurred to me that we could roll down the car windows, and when we did so we discovered that it was unusually cool out (almost chilly!), so I figured the cooler temperatures could be postponing any ant activity.

In all, as morning temperatures started to rise we wound up getting to watch a small swarm form at one of the better collecting spots, and managed to collect 59 queens in the end. We didn't see signs of any other swarming activity elsewhere, so I think this rain wasn't quite sufficient to really get things going. This past winter was extremely dry, so I'm hoping that overall this rainfall event will encourage plant growth and facilitate future flights, so long as future storms roll through. Monsoon rains are always a gamble.

Two photos:

The dark spots on the upper left are the leafcutter ant queens and males in midair. The ants fly towards an aggregation, where the males grab onto the queens while in flight, and then they fall to the ground and mate.
Swarn in the sky

Ants mating beneath the swarm:
Mating beneath the swarm

In this species, the queens mate multiple times, so after they've finished with one male, they pause for a millisecond and then take off again, back into the swarm. Quite a frenzy. We wanted to wait until we were sure the queens had mated as many times as they desire, so that meant waiting until it looked like the queens could no longer fly and were starting to shed/tear off their wings. Altogether we used those 59 queens to set up 20 nests of 3 queens per nest:

Housed

That isn't enough nests for everything we'd like to get done this year, so we'll continue to keep an eye on the weather. Ideally we'll manage to start up around 85 nests total, so that's 255 queens, at least. A fraction of the nests will inevitably die; over 95% of the queens in the wild die during the colony initiation phase. We tend to have better success in the lab, but some extra collecting never hurts. We shall see.

Progress

May. 18th, 2016 10:17 am
rebeccmeister: (Acromyrmex)
This is a bit convoluted because I'm still working on how to pack meaningful parts of the information below into the leafcutter manuscript (or how to avoid having to pack them in).

A challenging part of writing about the leafcutter manuscript involves how different groups of people think about and write about nutrition. From a broad ecological perspective, it makes the most sense to use elements as a fundamental currency, and that's the perspective I started from originally. For those interested in animal nutrition, however, it makes more sense to think about elements in nutrient form, and to distinguish between things that can be readily digested and assimilated or not. So I have to be more nutrient-explicit than just talking about elements.

But where do fungi fit in? Many kinds of fungi can break down cellulose and lignin, which is why fungi are key for decomposition. I learned yesterday that in many ecosystems, decomposition often occurs in a two-stage process, with small soil organisms initially breaking down leaves into small particles, and then fungi can come in and colonize things: when tough leaves are put into a mesh bag with pores that are so small that only microbes can enter, the leaves don't break down. As soon as the pores are large enough to permit entry of soil arthropods, the leaves get decomposed. On the other hand, if leaves that are easy to break down are put into those same mesh bags (e.g. kale), there's no difference in decomposition rates between mesh bag types. But the jury's out with regards to the extent to which cellulose digestion is important in the leafcutter system specifically.

Anyway. Over the course of further extensive literature searching, I also determined that next to nothing is known about the ability of fungi to ingest and utilize lipids as an energy source. The vast majority of work has focused on the utilization of different carbohydrate sources. Typically, glucose is prioritized, but in mixtures, fungi (in general) will use different blends of things. This corresponds well with some of the studies that have been conducted on enzyme activities in leafcutter fungus gardens - gardens will shift enzyme production in response to changes in fungal substrate.

I got to thinking about all of this over the course of trying to determine why, from a biochemical standpoint, there are large differences in the carbon and nitrogen content of palo verde leaves as compared to polenta. I think the main reasons are because corn is mostly full of stored carbohydrates, whereas leaves are full of photosynthetic machinery. In addition, leaves of desert plants often have more waxy cuticles to prevent water loss. Waxes and and chlorophyll are high in carbon, and chlorophyll is somewhat high in nitrogen, so these are reasonable explanations for the differences.

It took a while to think this through and find good references for it, though, because I wasn't sure about how large the differences in N content were for leguminous vs. non-leguminous plants, or whether the differences are strongly tied to the production of plant secondary compounds. I also had to remember which kinds of plants use C3 versus C4 versus CAM photosynthesis. The literature informs me, however, that at least ~75% of the protein in plant leaves in general is associated with chlorophyll, so on a coarse level this explanation seems like it will hold unless a reviewer informs me otherwise.

I'm still not sure about how to justify my diet treatments, though, or if I even need to do much in the way of justification. I do, however, need to be able to talk about why it is that colonies in the wild collect foods that have more protein-biased ratios than the high-protein polenta treatment I used.

The answer really lies in the waste material. Unfortunately, I haven't been able to analyze the waste material for anything other than overall elemental composition. I also need to go back to working on comparisons of throughput rates for leaves versus polenta. Just knowing elemental composition doesn't reveal the full story in terms of nutrient utilization; I need to know things about both input rates and output rates. The main thing I know at the moment is that they aren't exactly steady-state in smaller colonies, which complicates things - there's variation in retention times within the fungus garden. If there was even a hint of steady-state rates, I could say, "Here's what goes in, here's what comes out, so here's what the ants and fungus and microbial community have used up."

I could design and conduct some really good experiments with what I know now, but I really don't have that luxury at the moment.
rebeccmeister: (Acromyrmex)
On Friday and today, I've been working on that leafcutter manuscript I mentioned. I've gotten all the way to the Discussion, but have been feeling stuck on the Discussion. What to talk about, at what length? How to structure the damn thing? My PhD advisor offered one clue, in the form of "talk about your results first, THEN the other literature," based on the material that's currently there under the label of Discussion, but I have still been hung up on something. How to structure it so it all hangs together as a coherent story? What's the most efficient way to bang out a Discussion for an academic paper? In writing about the subject, I tend to wander off into the forest, admiring all the different trees and flowers, reading all the papers that are only remotely related to what I'm working on, and then reading all the interesting papers that are cited in those remote papers. Basically.

Just now, I had a flash of insight, based on something clever I learned from my first postdoc advisor. His strategy is to sketch out the main talking points based around the figures. Bring it back to the data, the heart of the story.

Duh.

I think I can do this now.
rebeccmeister: (bikegirl)
Ahh, bike maintenance. It always seems like a thing that can be scheduled in a straightforward fashion. Earlier in the week, the housemates and I were talking about how projects happen, and I noted that it wasn't a REAL project unless it involved at least two trips to the hardware store.

Bike maintenance usually doesn't require multiple trips to the bike shop, but it can require the occasional unanticipated trip, and regardless, it almost always takes twice as long, if not longer, to do.

On top of that, it has continued to rain here, a soft and gentle drizzle that mostly serves to make the indoors seem that much more cozy and outdoor activities slightly less enticing. [livejournal.com profile] sytharin did manage to get out during a gap in the clouds to turn the compost heap and also to trim a good 6-8 inches of dead, frizzed-out hair off my head.

Once that was accomplished, there was no more delaying the inevitable. I got the Jolly Roger all half-assedly washed up out on the back patio (poor bike, so grimy). I'd pulled off all the old brake pads before I realized that I only had a single set of spares in the bike parts bin. Thankfully, the Missing Link in Berkeley is open on Sundays. Also thankfully, Froinlavin is in rideable condition. It would have been highly irritating to further postpone the project.

S was kind enough to put up with me continuing to fuss around with bike parts during our Skype-Scrabble game. Adjusting brakes is not one of my favorite activities. V-brakes can be finicky.

It was dark by the time I got everything back together, and still raining, and time for dinner, so I haven't been out for a test ride yet. But at least it feels like I've accomplished a few things for the day's work.
rebeccmeister: (Acromyrmex)
Short one first, a time-lapse video of a leafcutter ant colony at the Houston Zoo demolishing a bouquet of roses:

https://youtu.be/AQ8-H8PZ3X8

Second, one of my colleagues from graduate school created a video to document the process used to make a cast of an ant nest. It's on the long side (15 minutes) but is thorough and cool.

https://youtu.be/6DXORV4ZflI
rebeccmeister: (Acromyrmex)
I visited my advisor this morning and we had a great discussion about the current leafcutter manuscript. Things are moving forward, hurrah! I am supposed to be working on revisions right this very minute so I can keep the momentum going. Hmm.

It was also fun to see the current state of the ants in the behavior lab. Here's my advisor and a kickass grad student in front of the Cabinet of Curiosities:

Fewell Lab Curiosity Cabinet
Blurry as usual. Thanks, smart-o-phone!

This student studies honeypot ants of the genus Myrmecocystus.

My photos came out mediocre or terrible, but here's an observation colony he constructed:
Myrmecocystus nest in the Fewell lab

The cool thing about these ants is that they use a subgroup of workers as living storage vessels for water, sugar, and fat.

Myrmecocystus nest in the Fewell lab

You can see some of the workers hanging from the ceiling with large, balloon-like abdomens.

This student was a huge help to me when he was an undergraduate because he and his father built a set of cabinets for this lab so I could more easily store all of my leafcutter ant colonies.

Apparently these guys were inspired after a visit to Germany last year/earlier in the year (date uncertain), to the Universität Würzburg, where they got to see some of the incredible nest designs partially featured in the film Ants - Nature's Secret Power. They returned to Arizona with ideas for how to improve their nests, and are now able to grow bigger, better seed-harvester colonies.

Pogonomyrmex nests in the Fewell lab

This is phenomenal for people whose research centers around colony size, colony organization, and colony growth.

Pogo nest design
rebeccmeister: (bikegirl)
I've wanted to make this graphic for several years by now:

HungryCaterpillarAnts

First, I had to draw pictures of individual worker ants - there are five different ant poses in this image. Then, I had to figure out how to extract the colors from the original Very Hungry Caterpillar: hand-position ants, one by one, on top of the caterpillar drawing, then merge them into a single image. Select all of the ants via a color threshold, then put the ant layer underneath the caterpillar layer, flatten the image, invert the selection, and press the 'delete' key.

Popular search engine searching-fu really didn't help at all for this one.

Noted here in case I or anyone else wants to employ this trick again.
rebeccmeister: (Acromyrmex)
1. Claremont is POSH.

2. The Biomathematics and Ecology Education and Research meeting is, once again, awesome. It's making me think about spending a bit of spare time taking some additional math courses. Also, I think you'd be surprised by the gender balance and level of diversity.

3. I'm thinking of some additional interesting modeling questions to pursue with my favorite pet biological system. I hope I'll figure out a way to do so. Hard when so much brainspace is getting eaten by crickets these days.

Work it

Sep. 30th, 2014 08:55 pm
rebeccmeister: (Acromyrmex)
Kind of a boring day, but for a good cause. I got a request to review a highly pertinent manuscript on Sunday night, but I have a lot on my plate at the moment, so this morning I decided to bite the bullet and got the review turned in.

I'm also giving a lot of talks in the next couple of weeks, beginning on Friday. Friday's talk is mostly done - it's an introduction to the software program R for an emerging technologies engineering grad student group on campus. It will be interesting to see the engineers' first impressions. Giving what amounts to an hour-long marketing pitch can be kind of tough, but it should be enough time to leave the graduate students with a good idea as to whether or not R will be useful for them.

Next week, it's an hour-long departmental seminar at my brother's university (ahh, sweet, sweet nepotism). Leafcutter-focused. Hopefully I will wow them all with my beautiful photos, excellent fieldwork and lab experiments, and superb graphs.

After that, a talk at a Bio-Math meeting, where the audience will be mostly mathematicians, and where I won't be talking about much math. My main goal is to get some behavioral datasets in sufficiently good condition that I can explain how they're generated and the opportunities they present so as to give the mathematicians more ideas for mathematical models of leafcutter ant colonies and behavior.

I might also be giving a talk to people in my grad research group the Monday after the Bio-Math meeting, which may or may not be the same as the departmental seminar. And a week or two after that, I'll go up to Taylor, Texas, for another department seminar. Theoretically these two department seminars will be good practice for any job talks I get to give, although I think it's going to be a while (if ever) before I have any such opportunity. More than anything, I hope I can manage to be articulate and not make a complete fool of myself. I hope my audiences come away inspired.

Oh, and there's a conference in November. That talk with be cricket-focused.

I want to be working on manuscript revisions of the respirometry manuscript, and on the draft of the Discussion for the next leafcutter manuscript, and on the cricket longevity manuscript. Sometimes the writing is a real slog.
rebeccmeister: (Acromyrmex)
First, have you yet read the Wired article about the new grasshopper species discovery? If you think it's tl;dr* watch the video first, then backtrack and read about this interesting line of work. It's somewhat relevant to me because I'm studying a wing-dimorphic cricket species - crickets are also Orthopterans, and "wing dimorphism" means that, within the species, there's variation in wing length and flight capability - as described in the Wired article, there have been important changes in flight capability in grasshoppers over evolutionary time.

Next, this piece on publicly prominent scientists should provide food for thought, regardless of how you feel about Richard Dawkins. It's pretty sad that most Americans don't know about the many other awesome women and minority scientists doing amazing things these days.

And lastly, I'll toot my own tiny horn briefly. I've updated my leafcutter ant research methods album over on flickr. I still feel I have a responsibility to the taxpaying public to share how I do the science that it funds. Besides, it's cool.



*too long; didn't read

Leafcutter

May. 16th, 2014 09:54 pm
rebeccmeister: (Acromyrmex)
I think I might finally be getting a handle on the statistics for this next leafcutter manuscript. Maybe. It's been a hard one to navigate because I've had to think things through without much feedback from anyone else. But that makes it a good self-test for my confidence in my abilities as a scientist. I just really don't want to embarrass myself when submitting this paper for publication. Oh, and I think I can potentially get it published somewhere spiffy.

The figures are beautiful, and the data tell an interesting story, especially with the addition of more contextual information on the protein and carbohydrate content of some plants that the ants collect in the wild. It's easier to justify the dietary treatments in the context of the protein-carbohydrate data than in the context of the elemental composition of a single leaf type we used to feed to the ants (how I'd originally framed things), especially since I've pulled out the other elemental data that were originally in the paper (for the leafcutter waste material). Elemental data can be useful, except in the case of carbon, because (a) most of the carbon in plants is locked up in non-digestible structures like cellulose, and (b) the carbon content of different compounds (cellulose vs. sugars) is basically the same, but those different compounds function in radically different ways in consumers.
rebeccmeister: (Acromyrmex)
Work today was a real grind.

No, seriously:
Today's work was a real grind

I've spent several hours grinding up all of the plant samples that we collected in Arizona. For larger plant samples, our lab has a cutter mill, but the cutter mill is messy and loses much of the material that is shoved into it. The leafcutter-collected plant samples are precious because they took a lot of work to collect, so I wanted to retain as much of the sample material as possible after grinding.

When I tried grinding a test sample several weeks ago, I had mixed success. I used some twiggy palo verde leaves, and while the leaves themselves powdered nicely, the small stems were nigh impossible to grind by hand. After a bit of research on Ye Olde Internet, I got the idea to try mixing my samples with dry ice:

Freeze-dried sample and dried ice

Crushing a Plant Sample

My arms are very tired now, after grinding up a mere 13 hard-won samples. But at least the job is done.

End result: powdered plant.

Yeah, not a perfect powder, but close enough.

Next, onward to measuring plant digestible protein and carbohydrate!
rebeccmeister: (Acromyrmex)
One of the greatest benefits of working with my current boss is that he's quite good at pinpointing ways to improve my academic writing, and we work well together as writers. Last week we met to discuss a current manuscript-in-progress, and he suggested spending a bit of time with two books while thinking about and working on revisions to the manuscript's introduction.

I had to spend the better part of last week working on two other manuscripts, so it wasn't until Friday afternoon that I cracked open one of the books, Physiological Ecology: How Animals Process Energy, Nutrients, and Toxins, by William Karasov and Carlos Martinez del Rio, and promptly found myself nodding to sleep. It's a well-written textbook, but I just couldn't focus, so I decided to cut my losses and work on sorting out the materials collected in Arizona instead.

Here are two examples:
IMG_3611

IMG_3615

Interesting stuff - in the first example, there are a bunch of mistletoe flowering/fruiting stems on the left-hand side. After looking at everything for a while, I determined that the tiny, seed-like items are actually mistletoe flowers.

Flipping my to-do schedule meant that I could go home at a reasonable hour and try to work on reading Physiological Ecology on Friday evening, instead. Of course, I didn't get as far as I would have liked. Hopefully I will manage to get myself to focus more today, instead.

--
Which brings me to the weekend. Saturday morning, the Aggies hosted the Lake Bryan Sprints. Originally, they'd hoped to rope in three teams, Baylor, St. Edwards, and UT-Austin, but Baylor and St. Ed's backed out at the last minute. It was great to have UT-Austin there, though, because the Aggies needed some good competition. UT won every event they entered, but A&M had some solid and very close (one-foot) second-place finishes, so there was a lot of good racing for everyone.

The A&M Open Women's 4+ had the same problem they always have - no one to race against - so I challenged them to a duel against me in the 1x at the very beginning of the regatta. They won, but not by a huge margin, and the race was good practice for all of us with keeping our heads in the game. After the 1x, I hopped in the launch and spent the rest of the morning aligning boats at the start and chasing after the boats as they raced.
--

The rest of the weekend mostly consisted of chores: groceries from Brazos Natural, a recycling haul and several more groceries from Village Foods, then cooking up some aged vegetables on Saturday night (note to self: don't buy discount eggplant from Farm Patch ever again, it's just not worth it). I played Pancake Factory on Sunday morning, although it's kind of a depressing game when I'm the only one to eat them. Then the sink and bathroom got good scrubbings, things got vacuumed, stuff that migrated across the house got migrated back, and the worst of the weeds got pulled in the garden. I have a feeling that the snails are going to eat almost everything I'm planting, but I just can't seem to make the time to give the garden a proper going-over.

I tried to invite people over for crafts on Sunday afternoon, but nobody made it, so I sat out back and worked on painting an oar until I got too cold and it was time to stop, and then I knitted a bit on a vest I will most likely frog, while C worked on crocheting some blanket squares.

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